Abstract Background The intracellular bacterium Listeria monocytogenes is an attractive vector for cancer immunotherapy as it can effectively deliver tumor antigens to antigen-presenting cells. leading to a robust antitumor response. Results In this study. we developed a novel vaccine platform called Listeria-based Live Attenuated Double Substitution (LADS). https://macorners.shop/product-category/memorial-charm-car-ornament/